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4 edition of Biologic properties of platelet-derived microparticles found in the catalog.

Biologic properties of platelet-derived microparticles

Biologic properties of platelet-derived microparticles

characterization of mechanisms of generation and adhesion.


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Published by National Library of Canada in Ottawa .
Written in English

Edition Notes

Thesis (M.Sc.) -- University of Toronto, 2001.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
Pagination3 microfiches : negative.
ID Numbers
Open LibraryOL19071459M
ISBN 100612631508

  Platelets were mainly associated with coagulation and hemostasis; however, other biological effects have been attributed to platelets, including angiogenesis, extracellular matrix synthesis, inflammation, and immune response. Dengue virus infection causes million cases of severe flu-like illness annually, escalating to life-threatening hemorrhagic fever or shock syndrome. Background. Due to the sustained morbidity and mortality that malaria-associated anaemia imposes on patients, malaria is still a global threat, most especially, to residents in sub-Saharan Africa. Merozoite invasion and destruction of erythrocytes, a target for this study, have been necessary due to its unique nature and also since the erythrocytes suffer the most brunt of malarial infection. he present study was undertaken to investigate whether, in severe sepsis, platelet-derived microparticles could produce reactive oxygen species through a phagocyte-type nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and if such particles may induce vascular cell apoptosis through a reactive oxygen species-dependent mechanism. Design Experimental study. Setting . Analysis of individual platelet-derived microparticles, comparing flow cytometry and capillary electrophoresis with laser-induced fluorescence detection. Analyst. Jun;(6) PubMed.

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Biologic properties of platelet-derived microparticles Download PDF EPUB FB2

The present chapter focuses on platelet-derived MP (PMP) characteristics, biogenesis, and biological roles. After a rapid overview of detection methods, PMP paradoxical functions in disease process and regenerative medicine are discussed, together with future prospects as diagnostic tools and therapeutic by: 5.

Platelet-derived microparticles (PMP) are a heterogeneous population of vesicles (Cited by: Platelet-derived microparticles Megakaryocyte-derived microparticles.

understand th e biology, composi tion, properties of microparticles in meningococcal sepsis. Blood. Platelet involvement in dissemination of tumor metastasis was first experimentally demonstrated several decades ago.

1 Potential mechanisms of the platelet effect on metastatic dissemination include PAR receptors, platelet‐derived lysophosphatidic acid and others.

2, 3 Platelets were previously shown to directly mediate tumor cell adhesion to the vascular wall thus facilitating their future Cited by: Strategy for analyzing the proteome of platelet-derived microparticles.

Microparticles were generated by activating platelets with the agonist, ADP, fractionated by centrifugation, and lysed with SDS. The resulting protein samples were frac-tionated by SDS-PAGE on a % gel that was then sliced into 26 bands of equal size.

All microparticles harbor cell-surface proteins and contain cytoplasmic components of their original cells. They exhibit negatively charged phospholipids, Biologic properties of platelet-derived microparticles book phosphatidylserine (PS), at their surface, which accounts for the procoagulative character and proinflammatory properties of microparticles, including the alteration of vascular function.

Platelet-derived microparticles (PMPs), small particles derived from the membranes of intact platelets also known as platelet-derived microvesicles, are present in PC. PMPs are strongly procoagulant, and there is evidence that they retain many of the biologic properties of intact platelets (see Chapter 22).

Various mechanisms may contribute to. Microparticles (MPs) are small membrane-bound vesicles that display potent biological activities that can have an impact on normal physiology as well as the pathogenesis of immune-mediated diseases. These particles range in size from to μm and arise from activated or dying cells.

Platelets and other cells release microparticles (MPs) into the plasma in response to receptor agonists and shear stress At least 45% of plasma-borne MPs are platelet-derived microparticles (PMPs)13, PMP release increases in individuals bearing solid tumors, but roles of PMPs in cancer progression are incompletely understood15, PMPs.

CHRISTOPHER LONGO, THOMAS W. WAKEFIELD, in The Vein Book, Microparticles. Microparticles (MP) Biologic properties of platelet-derived microparticles book small (less than 1 micrometer, about the size of a bacterium) phospholipid vesicles that are shed from a variety of cell types including platelets, leukocytes, and endothelial cells.

42–44 Microparticles are a normal constituent of blood and can be isolated from plasma by. One important property of platelet-derived microparticles (PMPs) is signal transfer, as seen in sepsis and heparin-induced thrombocytopenia (HIT) [2,3].

PF4 is a platelet-specific heparin-binding protein that is abundant in platelet alpha granules from which it is secreted following platelet stimulation. Abstract. Platelet microparticles are submicron vesicles that can support thrombin generation on externalized negatively charged phospholipids.

Increased numbers of circulating platelet microparticles have been investigated as the basis of hypercoagulability in a variety of prothrombotic conditions. Platelet-derived microparticle levels are significantly elevated in patients treated by elective stenting compared to subjects with diagnostic catheterization alone.

Reports in the last decade have suggested that the role of platelets in atherosclerosis and its thrombotic complications may be mediated, in part, by local secretion of platelet-derived microvesicles (pMVs), small cell blebs released during the platelet activation process.

MVs are the most abundant cell-derived microvesicle subtype in the circulation. Rationale: Obesity is a risk factor for atherothrombosis and various cancers. However, the mechanisms are not yet completely ives: We aimed to verify whether the microparticles (MPs) released from thrombin-activated platelets differed in obese and non-obese women for number, size, and proteomics cargo and the capacity to modulate in vitro the expression of (i) genes related to.

Properties of membrane microparticles (MPs), as well as methods for their study are reviewed. Microparticles are vesicular fragments of a plasma membrane, which are detached from the surface of cells upon their activation and/or damage.

An increase in intracellular calcium and subsequent remodeling of membrane cytoskeleton and redistribution of membrane phospholipids are key events. The total platelet-derived MP count per μL, assessed by cytofluorimeter, was not significantly different in non-obese and obese individuals ( ± vs.

±respectively). We studied the biophysical light scatter properties of MPs obtained in. Microparticles. Physiologically, cells form MP during activation and aging but MP formation also occurs in pathological conditions, e.g.

in response to hypoxia, irradiation, oxidative stress, exposure to proteins of the complement cascade and shear stress [1–8].MP circulate within the peripheral blood with an average concentration of 5–50 μg/ml. 2 Microparticles: Definition and genesis.

MPs fall under the umbrella term of extracellular vesicles (EV), which is used to designate all types of vesicles released from cells, the best characterized being exosomes, apoptotic vesicles (also called apoptotic bodies), and MPs [].These various types of EV differ in regards to several characteristics such as size, density, morphology, composition.

The annexin V binding properties of platelet-derived microparticles: Medicine & Health Science Books @ Comparison of anticoagulant and procoagulant activities of stimulated platelets and platelet-derived microparticles.

Blood. ; – Medline Google Scholar; Perez-Casal M, Downey C, Fukudome K, Marx G, Toh CH. Activated protein C induces the release of microparticle-associated endothelial protein C receptor.

Blood. ; The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles. Journal of Biological Chemistry(20 Biochemical properties of platelet microparticle membranes formed during exocytosis resemble organelles more than plasma membrane.

Platelet-Derived Microparticles in Patients with Arteriosclerosis. Role of cell-derived microparticles in the pathogenesis of MS. Endothelial and platelet-derived microparticles normally circulate in plasma, and their numbers increase during inflammatory and thrombotic diseases (Lowery-Nordberg et al., ).

These tiny fragments of the cell membrane of their parent cells, vary in size from to 1 μm. The. These biological microparticles are thought to be shed from the plasma membrane of the cell as lipid bilayer-bound entities that are typically larger than nm in diameter.

"Microparticle" has been used most frequently in this sense in the hemostasis literature, usually as a term for platelet EVs found in the blood circulation.

Connor DE, Exner T, Ma D, et al. The majority of circulating platelet-derived microparticles fail to bind annexin V, lack phospholipid-dependent procoagulant activity and demonstrate greater expression of glycoprotein Ib.

Thromb Haemost. ; – Platelets play a crucial role in the maintenance of hemostasis, as well as in thrombosis.

Upon activation, platelets release small membrane-bound microparticles (MPs) containing bioactive proteins and genetic materials from their parental cells that may be transferred to, and exert potent biological effects in, recipient cells of the circulatory system.

Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood. ; – Crossref Medline Google Scholar; 15 Lindemann S, Tolley ND, Dixon DA, McIntyre TM, Prescott SM, Zimmerman GA, Weyrich AS. Activated platelets mediate inflammatory signaling by regulated interleukin 1β synthesis.

Platelet-derived microparticles (PMP) participate in blood coagulation, whereas MP secreted by skeletal cells initiate bone mineralization, and MP secreted by normal endothelial cells have been implicated in angiogenesis.

5 It was shown that the effect of endothelial-derived MP on angiogenesis is dose-dependent and that whereas endothelial. karyotic cells. After adhesion to target cells, MP may transfer membrane-associated proteins to these cells. We found that peripheral blood platelet- (PMP) and megakaryocyte-derived MP (MegaMP) that highly express CXCR4 may transfer this receptor from the surface of platelets or megakaryocytes to the surface of CXCR4-null cells.

Design:Since this mechanism could potentially allow CD4+/CXCR4. Because human CD34 + and murine Sca-1 + hematopoietic stem–progenitor cells (HSPCs) express platelet-binding sialomucin P-selectin (CD) and integrin Mac-1 (CD11b–CD18) antigen, it was inferred that these cells might interact with platelets.

As a result of this interaction, microparticles derived from platelets (PMPs) may transfer many platelet antigens (CD41, CD61. These thrombocyte derived microparticles contain glycoproteins receptors as that of thrombocytes. These receptors can be transfered to other cell surfaces. Platelet derived micro-particles (PMPs) plays critical function in the haemostasis and thrombosis.

we can discourse the features of PMPs and its function in the disease provinces. Platelet‐derived microparticles (PMP) constitute the majority of the pool of MP circulating in the blood 5. PMP contain a unique subset of proteins derived from the parent cell, and in recent years, it has become clear that PMP have important biological functions.

In recent years, the field of transfusion medicine has seen increased study of cell-derived microparticles (MPs). A number of studies have demonstrated the presence of MPs in packed red blood cells, platelet concentrate, and fresh frozen plasma in storage.

1–3 Other studies have suggested the presence of procoagulant activities of these MPs through the expression of tissue factors and.

Circulating microparticles (MPs) are –1-μm-large phospholipid vesicles 1 released from blood and vascular cells upon activation and apoptosis.

The mechanism of MP formation by budding of the outer cell membranes provides them with procoagulant activity, mainly due to phosphatidylserine exposure and tissue factor expression 2, factor-bearing MPs are important for thrombin.

Introduction. Microparticles (MP) are submicron vesicles (size range, –1 μm) released from cell membranes in response to activation or apoptosis. 1 They generally expose phosphatidylserine and membrane antigens representative of their parent cells.

2 MP behave as vectors of bioactive molecules, involved in various biological responses. 3 – 5 Circulating MP are elevated in many. Microparticles (MPs) are a group of small spherical membranous vesicles. The structure and cellular origin of MPs, mechanisms that stimulate their secretion and the place of their production, determine their biological properties, which could become manifest in the pathogenesis of immune-mediated inflammatory diseases.

Janowska-Wieczorek et al 58 showed that platelet-derived microparticles stimulate mitogen-activated protein kinases in lung carcinoma cell lines and increase cell proliferation. Further, incubating A lung carcinoma cells with the microparticles led to increased expression of matrix metalloproteinases (MMPs) and increased invasion through.

The present study was undertaken to investigate whether, in severe sepsis, platelet-derived microparticles could produce reactive oxygen species through a phagocyte-type nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and if such particles may induce vascular cell apoptosis through a reactive oxygen species-dependent mechanism.

Platelet-derived microparticles (PMPs), the most abundant constitutive microparticles in the blood circulation, are small vesicles with a diameter less than 1 mm. PMPs typically are shed from.

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The most important physical property of platelets is the maintenance of Hemostasis, and it depends on three factors. The endothelial supporting function of platelets, The ability to form hemostatic plugs and ; To release lipoprotein material (platelet factor 3). Platelet-derived microparticles (PDMPs), insofar as they display membrane surface antigens characteristic of activated PLTs, may participate in coagulation and thrombogenesis.

(3,9,10) One such antigen, CD42b (GPIb[alpha]), is part of the CD42a-d complex that serves as a receptor for von Willebrand factor and thrombin.Platelet-Derived Microparticles in the Systemic Circulation and in Pericardial Blood. To investigate the presence of platelet-derived microparticles in the circulation of patients undergoing coronary bypass surgery, blood was collected as described in “Methods” and analyzed by whole blood flow cytometry.